Tapinder Kaur Grewal, Shahnawaz Majeed and Sadhna Sharma* Pages 135 - 146 ( 12 )
Background: Despite the advancement in the development of newer anti-HIV drugs, various drawbacks still persist with antiretroviral therapy. Therefore, this study was conducted to overcome the pharmacokinetic limitations of commonly prescribed anti-HIV drugs with the implication of nanotechnology.
• Formulation of nanoparticles carrying antiretroviral drugs.
• Pharmacokinetic and pharmacodynamic study of nanoparticles loaded with antiretroviral drugs viz zidovudine (AZT), didanosine (ddI), and ritonavir (RTV).
• Ex vivo study of nanoparticles loaded with antiretroviral drugs.
Methods: Poly (lactide-co-glycolide) (PLGA) nanoparticles encapsulating AZT, ddI and RTV were developed by multiple emulsion solvent evaporation technique and characterized for size, shape, zeta potential, polymer drug interaction etc. Comparative pharmacokinetic studies were carried out following single oral dose administration of free or nano drugs in mice.
Results: PLGA nanoparticles loaded with HIV drugs were found to be stable and in nanorange. FTIR images of ddI and RTV did not show any interaction of these drugs with polymer except for AZT. The absence of any sharp diffraction peaks in the XRD of encapsulated drugs indicated complete amorphization of drugs in nanoparticles. The pharmacokinetic parameters were significantly improved upon nanoencapsulation as compared to the parent drugs. Fluorescent nanoparticles were found to be efficiently taken up by peritoneal macrophages and cytotoxicity assay indicated that PLGA nanoparticles are safe to be used as drug delivery system.
Conclusion: PLGA nanoparticles exhibited sustained release of AZT, ddI and RTV in mice with improved pharmacokinetic parameters and hold great prospective for extended research in higher animals.
Zidovudine, didanosine, ritonavir, PLGA nanoparticles, anti-HIV drugs, antiretroviral drugs.
Department of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh –160012, Department of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh –160012, Department of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh –160012