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Phyto-Phospholipid Complex of Eclipta alba Extract Containing Coumestans for the Management of Hepatotoxicity

[ Vol. 7 , Issue. 3 ]

Author(s):

Abhishek K. Jain, Awesh K. Yadav, Shweta Kumar, PK Laxmi and Rajesh S. Pawar   Pages 212 - 222 ( 11 )

Abstract:


Background: Eclipta alba Hassk. (Family: Compositae) contains coumestans useful in liver problems. The present work is aimed to develop a new formulation of plant-derived coumestans in combination with the soya-phosphatidylcholine (PC) and it is expected to improve the absorption of coumestans. The study also examines the protective effect of coumestans-phosphatidylcholine complex (C-PC) on carbon tetrachloride (CCl4) induced acute liver injury in rats.

Method: Coumestans (C) were characterized in the ethyl acetate fraction of methanolic extract (EFME). The C-PC was characterized by DSC and FTIR spectroscopy. In vitro drug release from EFME and C-PC through egg-membrane was measured using a UVVisible spectrophotometer. The hepatoprotective activity of C-PC (equivalent to 5.35 and 10.7 mg/kg body weight of EFME), ME 250 mg/kg and EFME 5.35 mg/kg was analyzed by assessing the level of various enzymes.

Conclusion: C-PC significantly (P<0.001) provided better protection to the liver by normalizing the levels of enzymes SGPT, SGOT, ALP and total bilirubin when compared to CCl4 group. The histopathological study showed that C-PC showed improvement in rat’s liver as compared to ME and EFME at similar doses as they showed regeneration of hepatocytes, central vein, intact cytoplasm and nucleus.

Keywords:

Soya phosphatidylcholine complex, CCl4, coumestans, hepatotoxicity, Eclipta alba, SGPT, SGOT, ALP.

Affiliation:

Pharmacognosy and Phytochemistry Division, Faculty of Pharmacy, VNS Group of Institutions, Bhopal, Madhya Pradesh, Bhagyoday Tirth Pharmacy College, Acharya Vidhya Sagar Marg, Sagar, MP, Pharmacognosy and Phytochemistry Division, Faculty of Pharmacy, VNS Group of Institutions, Bhopal, Madhya Pradesh, Pharmacognosy and Phytochemistry Division, Faculty of Pharmacy, VNS Group of Institutions, Bhopal, Madhya Pradesh, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, VNS Group of Institutions, Bhopal, Madhya Pradesh

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